One medium was bairdparker agar base supplemented with egg york. Baird parker agar medium is formulated on the principle that staphylococci are able to reduce tellurite to tellurium and to detect lecithinase from. The purpose of this project was to evaluate the use of 2 selectivedifferential culture. The cofa will be sent to you by email with 1 to 3 business days. Use with ey tellurite enrichment for the detection and enumeration of coagulase positive staphylococci in foods and other materials. In 1962, bairdparker developed his own medium based on zebovitzs telluriteglycine. Baird parker agar base, criterion dehydrated culture media, 500gm widemouth bottle, by hardy diagnostics. The medium may also be used for identification of staphylococci on the basis of lecithinase activity. Also the selective mediumbairdparker agar was used for the isolation of. English description baird parker agar base is a selective medium used with supplements for the isolation and enumeration of staphylococcus aureus from food, foodstuffs and water. Bairdparker agar is a selective medium for the enumeration of staphylococcus aureus in foods and this was first reported by bairdparker. Hardy diagnostics criterion bairdparker agar base is used with egg yolk tellurite enrichment as a selective medium for the detection and enumeration of coagulasepositive staphylococci from food samples. Page 1 of 7 sds 7112 rev 0 march 2016 section 1 identification of the substancemixture and of the company 1.
Baird parker agar 500 g 306200061 standard bioworld. Baird parker agar is a selective medium for the isolation and presumptive identification of coagulasepositive staphylococci. Bp agar is used for detection of staphylococcus aureus in food, dairy products and cosmetic products. Detection of staphylococcus aureus in bulk tank milk using modified. Baird parker agar is used with egg yolk tellurite enrichment for the detection, isolation and enumeration of coagulase positive staphylococci in foods and cosmetics.
Principle tryptone and beef extract are a most satisfactory source of nitrogen and contain a mix of peptides and free amino acids. Baird parker agar culture media online microbiology notes. Bairdparker added sodium pyruvate, to protect damaged cells and aid their recovery 2 and egg yolk emulsion as a diagnostic agent. The medium has the advantage of considerably reducing the number of confirmation tests for the presence of coagulase positive staphylococci. Browse dehydrated media products and more laboratory tools. The oxoid bairdparker rpf agar base is a selective medium for the isolation and enumeration of coagulase positive staphylococci from food. Evaluation of two chromogenic agar media for recovery and. Baird parker agar b02102 safety data sheet according to 29 cfr 1910. Baird parker agar base rpf for microbiology sigmaaldrich. Egg yolk is the substrate to detect lecithinase production and lipase activity. Use fom below to request a certificate of analysis. It is a partially selective medium which applies the ability of staphylococci to reduce tellurite to tellurium and to detect lecithinase from egg lecithin.
Baird parker agar base selective medium for detection and enumeration of coagulasepositive staphylococci in food and animal feed, according to iso 6888. Find the baird parker agar detection and enumeration of staphylococci at weber scientific online. This medium is a modification of baird parker medium and is recommended for the selective isolation, enumeration and confirmation of staphylococcus aureus from food and other specimens 3. Easy interpretation of test results as staphylococcus aureus forms greyblack shiny colonies with surrounding clear zones. A selective diagnostic medium for the isolation and presumptive identification of staphylococcus aureus in a contact plate to permit direct environmental sampling. Detection of staphylococcus aureus in bulk tank milk using. It contains lithium chloride and tellurite to inhibit the. Baird parker medium was developed by baird parker to isolate and enumerate coagulasepositive staphylococci from foods. Bairdparker agar was developed in 1962 for isolation and enumeration of coagulasepositive staphylococci from foods and other materials. Plates should be used on the same day of preparation or within 48 hours, to avoid the loss of definition in the precipitated zones.
Bairdparker 1 developed this medium from the telluriteglycine formulation of zebovitz et al. Staphylococcus selective agar, staphylococcus selective agar acc. Baird parker agar was developed by baird parker 1, 2 from the telluriteglycine formulation of zebovitz et al 3 for isolation and enumeration of staphylococci in food and other material since it allows a good differentiation of coagulase positive strains. Bpa rpf, baird parker rpf agar, rpf rabbit plasma fibrinogen. This medium is a modification of bairdparker medium and is recommended for the selective isolation, enumeration and confirmation of staphylococcus aureus from food and other specimens 3. P16 is not intended to be used for the diagnosis of human disease. Bairdparker rpf agar intended use baird parker rpf rpf rabbit plasma fibrinogen agar is used for the direct detection and enumeration of coagulase positive staphylococci. The chapter discusses bairdparker agar composition, which consist of tryptone, beef extract, yeast extract, lithium chloride, sodium pyruvate, glycine, potassium. The identification on bairdparker culture medium represents the gold. Assessment of the coagulase test in the identification of. Lithium chloride and potassium tellurite act as selective agents. The chapter discusses bairdparker agar composition, which consist of tryptone, beef extract, yeast extract, lithium chloride, sodium pyruvate, glycine, potassium tellurite, sulphamezathine, egg yolk emulsion, agar, and distilled or deionized water. Baird parker agar was developed by baird parker from the telluriteglycine formulation of zebovitz et al for isolation and enumeration of staphylococci in food and other material since it allows a good differentiation of coagulase positive strains. Home type contact plate bairdparker agar, contact plate.
An improved diagnostic and selective medium for isolating. Dehydrated microbiology culture media cultivate and isolate microorganisms for researching purposes. Baird parker agar was developed by baird parker 4,5 from the telluriteglycine formulation of zebovitz et al 16 for isolation and enumeration of staphylococci in food and other material since it allows a good differentiation of coagulase positive strains. Coagulasepositive staphylococcus selective isolation. Bairdparker agar is a type of agar used for the selective isolation of grampositive staphylococci species. It is a moderately selective medium for the isolation and differentiation of coagulase positive. Although almost all of the scvs grew on the chromogenic agar media, they did not exhibit a change of color. Pb20 our bairdparker agar is used for the selective isolation and differentiation of coagulasepositive staphylococci from food and other nonclinical sources. It is widely recommended by national and international bodies. Created using powtoon free sign up at create animated videos and animated presentations for free.
Baird parker agar detection enumeration weber scientific. It contains lithium chloride and tellurite to inhibit the growth of alternative microbial flora, while the included pyruvate and glycine promote the growth of staphylococci. Medium for the preparation of eggtelluriteglycinepyruvate agar bairdparker agar which is used for the selective isolation and enumeration of coagulasepositive staphylococci. Baird parker agar is used for the selective isolation and enumeration of coagulasepositive staphylococci. For the selective isolation of coagulasepositive staphylococci. Baird parker agar principle, preparation and uses learn. Principle pancreatic digest of casein and meat extract provide amino acids, carbon, nitrogen, vitamins and minerals. Baird parker 1 developed this medium from the telluriteglycine formulation of zebovitz et al. Bairdparker agar for in vitro use only catalogue no. Composition, principle, preparation, result interpretation, uses and limitations of baird parker agar. Culture media used in microbiology archives page 2 of 3. Baird parker agar commonly used in the detection and enumeration of. For bairdparker medium lab285 with rabbit plasma fibrinogenrpf supplement x086 weigh 6.
The rpf agar formulation retains the bairdparker agar base which has been specifically formulated to resuscitate injured cells 4. Baird parker added sodium pyruvate, to protect damaged cells and aid their recovery 2 and egg yolk emulsion as a diagnostic agent. Baird parker rpf rpf rabbit plasma fibrinogen agar is used for the direct detection and enumeration of coagulase positive staphylococci. Baird parker agar is used for the isolation and differentiation of coagulase positive staphylococci in food and pharmaceuticals according to baird parker. Composition ingredients gms litre pancreatic digest of casein 10. It is primarily used in processing of food, cosmetics and environmental samples rather than clinical samples. Firefighting measures continued as in any fire, prevent human exposure to. Bairdparker agar base is widely recommended by national and international bodies for the isolation of staphylococcus aureus 1. The formulation of the present bairdparker agar was published in 1962. One medium was bairdparker agar base supplemented with egg york tellurite emulsion and acriflavine.
Bairdparker agar medium mu043 bairdparker agar medium is recommended for the selective isolation and enumeration of coagulase positive staphylococci from food and other materials in accordance with united states pharmacopoeia. A selective diagnostic medium for the isolation and presumptive identification of staphylococcus aureus from foodstuffs. The purpose of this project was to evaluate the use of 2 selectivedifferential culture media for detecting staphylococcus aureus in bulk tank milk. Staphylococcus aureus and staphylococcus epidermidis on baird parker agar. Bairdparker agar, 90mm plate southern group laboratory. It is a selective medium for the isolation and presumptive identification of coagulasepositive. Hardy diagnostics bairdparker agar is a selective medium for the detection and enumeration of coagulasepositive staphylococci from food samples.
Bd bairdparker agar is a moderately selective and differential medium for the. Bairdparker agar is a selective medium for the enumeration of staphylococcus aureus in foods and this was first reported by baird parker. Bairdparker agar base is used with ey egg yolk tellurite enrichment in the preparation of eggtelluriteglycerinepyruvate agar etgpa for selective isolation and enumeration of coagulasepositive staphylococci from food, skin, soil, air, and other materials. The book is quite typical of the volumes of poetry published in, say, the past two years. Selectivity is attained with potassium tellurite and lithium chloride. To identify the most rapid and reliable technique for recovery and identification of staphylococcus aureus small colony variants scvs, the colonial appearance of 106 isolates representing scvs and the normal phenotype were evaluated on two newly described chromogenic agar media. Bairdparker agar bp agar is a selective medium for identification and enumeration of coagulase positive staphylococcus spp.
1353 1529 776 1189 1553 701 369 1461 760 66 1535 939 1433 1525 981 958 1272 607 1210 422 771 21 486 278 1401 276 1132 765 734 355 320 813 427 1529 1375 185 334 526 798 372 1422